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Anti-Clathrin heavy chain antibody [TD.1] (ab24578)反網絡蛋白重鏈抗體產品信息：
- Product nameAnti-Clathrin heavy chain antibody [TD.1]See all Clathrin heavy chain primary antibodies ...
- Description
Mouse monoclonal [TD.1] to Clathrin heavy chain
- SpecificityThis antibody reacts with clathrin heavy chains (CHC).
- Tested applicationsIHC-P, WB, ICC/IF
- Species reactivity
Reacts with: Cow, Human
- Immunogen
This antibody was raised using the globular N terminal domain of clathrin heavy chain.
- Positive control
- Methanol fixed HeLa cells for IF.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.03% Thimerosal (merthiolate).
Constituents: PBS. Concentration 100 µl at 1 mg/ml
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberTD.1
- IsotypeIgG1
- Research Areas
- Signal Transduction
- Protein Trafficking
- Vesicle Transport
- Coat Proteins
- Tags & Cell Markers
- Subcellular Markers
- Organelles
- Caveolae and Clathrin
Applications
Our Abpromise guarantee covers the use of ab24578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use at an assay dependent concentration. PubMed: 21571834 |
| WB | WB: 1/250. Predicted molecular weight: 192 kDa. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
- Application notesIs unsuitable for or IP.
Target
- FunctionClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
- Sequence similaritiesBelongs to the clathrin heavy chain family.
- Cellular localizationCytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
Database links
- Entrez Gene: 281080 Cow
- Entrez Gene: 1213 Human
- Omim: 118955 Human
- SwissProt: P49951 Cow
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- Unigene: 491351 Human
Alternative names
- CHC17 antibody
- Clathrin heavy chain 1 antibody
- Clathrin heavy chain 2 antibody
Anti-Clathrin heavy chain antibody [TD.1] images
![Immunocytochemistry/ Immunofluorescence - Clathrin heavy chain antibody [TD.1] (ab24578)](http://a.abcam.com/ps/datasheet/Images/24/ab24578/ab24578_1.jpg)
Immunocytochemistry/ Immunofluorescence - Clathrin heavy chain antibody [TD.1] (ab24578)
ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
公司介紹：
Abcam位于英國的劍橋科學園，成立于1998年，專門生產和分銷研究型抗體。主要創立人Jonathan Milner和實驗室同事構思成立一間了解科研人員需要的網上抗體公司，理念是Abcam銷售質量高的抗體，并提供zui全面、誠實、時新的說明書，快速的送貨服務，及高效率的顧客和服務。我們的在線目錄(m.jlcgcl.com) 已有差不多100,000種抗體和試劑，并不斷添加，供應予百多個國家。Abcam于2005年11月在倫敦證券交易所上市，在美國馬薩諸塞州、日本東京及香港均設有分公司。
Abcam 一直致力加強產品線，為使研究員更容易找到蛋白質研究試劑產品，在2011并購了美國的MitoSciences公司，加強了免疫分析方面的產品供應；同年也并購了英國的Ascent Scientific 公司，開展了生化試劑的供應。在2012年，Abcam并購了美國的Epitomics 公司，成為一家有的RabMAbs® 供應商。
Abcam 的 目標是給世界上的抗體建立zui大的在線目錄，為各地科學家提供*產品，成為各國科學界的重要伙伴。我們為所有產品提供來使客戶獲得預期的結 果。為提供高質量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應用和物種中檢測每種抗體。我們相信誠信才是上策，在我們的上會發布有關我們 每一種產品的盡可能多的信息。
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Abcam ab1266Goat polyclonal to HA tag （FITC）250ug3996HA taghystemcell一抗
Abcam ab1267Goat polyclonal to Glu-Glu tag （HRP）100ug3996Glu-Glu taghystemcell一抗
Abcam ab12675Rabbit polyclonal to GPCR MCH250ug3996GPCR MCH2hystemcell一抗
Abcam ab12678Rabbit polyclonal to GPR 17950ug4940GPCR AGR9hystemcell一抗
Abcam ab1268Goat polyclonal to Glu-Glu tag （FITC）250ug3996Glu-Glu taghystemcell一抗
Abcam ab12680Rabbit polyclonal to D650ug3996GPCR D6hystemcell一抗
重慶市華雅干細胞技術有限公司